T. Karimi Jalilehvandi; S. Maleki Farahani; A.R. Rezazadeh
Abstract
In order to investigate the effect of sowing date and nitrogen and phosphorus fertilizer on the seed flavonoid content, qualitative and germination characteristics of lady’s mantle (Lallemantia royleana Benth.), a factorial experiment based on randomized complete block design with three replications ...
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In order to investigate the effect of sowing date and nitrogen and phosphorus fertilizer on the seed flavonoid content, qualitative and germination characteristics of lady’s mantle (Lallemantia royleana Benth.), a factorial experiment based on randomized complete block design with three replications was conducted in 2013-2014 crop year. Experimental factors included sowing date at two levels (autumn and spring) and chemical fertilizer at three levels (without fertilizer, half application of required fertilizer (23 kg ha-1 pure N+ 50.6 kg ha-1 P2O5) and full application of required fertilizer (46 kg ha-1 pure N+ 101.2 kg ha-1 P2O5). Flavonoid measurement of the seeds was performed weekly from flowering to seed ripening. The results of the mean comparison showed that the highest seed germination percentage was obtained at autumn sowing (74.8%) with an increase of 11.6% compared to spring sowing and at the full application of required fertilizer (88.6%) with an increase of 73.4% compared to without fertilizer. The application of chemical fertilizer and autumn cultivation of lallemantia significantly increased the percentage of seed mucilage compared to control. Seven days after flowering, the highest flavonoid content was obtained at spring sowing (0.264 mg quercetin per g sample) with an increase of 63.97% compared to autumn sowing, and at no fertilizer treatment (0.282 mg quercetin per g sample) with an increase of 90.54% compared to the full application of required fertilizer (0.148 mg quercetin per g sample). Twenty-eight days after flowering, the highest flavonoid content was obtained at spring sowing (0.554 mg quercetin per g sample) and no fertilizer treatment (0.629 mg quercetin per g sample). In general, the results showed that the autumn sowing date and full application of required chemical fertilizer increased the seed quality in terms of mucilage percentage and germination, but the spring sowing date and no fertilizer treatment increased the flavonoid content of lallemantia seeds.
S. Anbarestani; A.R. Rezazadeh; A. Rezaei
Abstract
Black cumin (Nigella sativa L.) is a medicinal plant belonging to the Ranunculaceae family. The seeds of Nigella sativa, also known as black seed, are used in traditional medicine as a natural remedy for several illnesses including asthma, inflammation, diabetes, and hypertension. The cell culture of ...
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Black cumin (Nigella sativa L.) is a medicinal plant belonging to the Ranunculaceae family. The seeds of Nigella sativa, also known as black seed, are used in traditional medicine as a natural remedy for several illnesses including asthma, inflammation, diabetes, and hypertension. The cell culture of this plant is important because of its active ingredients and significance in medicine. In this research, the effects of fungal elicitor (zero, 0.5 and 1 mg/L), and sucrose (30, 45 and 60 g/L) on the cell culture of black cumin was investigated as a factorial experiment in a completely randomized design with three replications. The cell growth characteristics and biochemical traits were assayed. The results showed that fungal elicitor and sucrose increased the membrane lipid peroxidation, protein concentration, phenol contents, hydrogen peroxide, and anthocyanin whereas peroxidase activity was significantly decreased compared to control. Generally, sucrose and fungal elicitor increased the growth and activity of the cell and increased the protein production by increasing oxidative stress in cells and increasing the entry of substances into the cell and stimulating metabolism.
T. Karimi Jalilehvandi; S. Maleki Farahani; A.R. Rezazadeh
Abstract
In order to evaluate the effects of autumn and spring sowing date and different amount of nitrogen and phosphorus fertilizer on qualitative characteristics and seed germination of Lallemantia (Lallemantia royleana Benth.), a factorial experiment in a randomized complete block design (RCBD) with three ...
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In order to evaluate the effects of autumn and spring sowing date and different amount of nitrogen and phosphorus fertilizer on qualitative characteristics and seed germination of Lallemantia (Lallemantia royleana Benth.), a factorial experiment in a randomized complete block design (RCBD) with three replicates was conducted in Medicinal Plant Research Center of the Shahed University during 2013-2014. The factors were sowing date at two levels (autumn and spring) and chemical fertilizer at three levels (without fertilizer, half dose of fertilizer (23 kg/ha N+ 50.6 kg/ha P2O5) and full doze of fertilizer (46 kg/ha N+ 101.2 kg/ha P2O5). Results for analysis of variance showed that the effect of sowing date on percentage of mucilage, germination percentage, mean germination time and germination rate, Catalase activity during grain filling, percentage of nitrogen and phosphorus was significant however on seed vigor index (SVI-II) was not-significant. The effect of chemical fertilizers on all traits was significant. The interaction effect of sowing date and fertilizer was not significant on all traits except the Catalase activity, percentage of nitrogen and phosphorus. Mean comparison showed that the highest seed germination percentage (74.815) was related to the autumn sowing and full amount of fertilizer (88.611). Regarding to the sowing date, the highest percentage of germination was related to the fall sowing (74.81) and regarding to fertilizer application, the full amount of fertilizer had the highest germination percentage (88.81). The highest catalase activity was 28 days after flowering in the autumn sowing and control (0.0075), and the lowest catalase activity was 7 days after flowering in spring planting and full dose of fertilizer (0.0014). For high germination indices, fertilizer and fall planting is recommended. Generally, results showed that fall sowing date and full dose of chemical fertilizer (46 kg/ha N and 101.2 kg/ha P2O5) produced seeds with higher seed vigor compared to other treatments.
